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香蕉线条病毒GF(Banana Streak Virus GF,BSGFV)是一种危害香蕉的主要香蕉线条病毒(Banana Streak Virus,BSV)，为高效检测该病毒，本研究根据BSGFV外壳蛋白(Coat Protein,CP)基因的保守序列设计特异性引物，建立了SYBR Green Ⅰ实时荧光定量PCR(Fluorescence Quantitative PCR,qPCR)方法．结果表明，qPCR检测特异性引物BSGFV-qG3F/qG3R的最适宜浓度为0.2μmol/L，最适退火温度为59℃．qPCR可以检测到最低质粒浓度为1.0×102 copies/μL，检测质粒的灵敏度是PCR的100倍，检测体系Ct值的变异系数小于5%．所建立的qPCR方法只适用于田间主栽品种如巴西蕉(AAA)等香蕉中BSGFV的检测，不适用于含B基因组的杂交种香蕉中BSGFV的检测．本研究为田间主栽品种香蕉中BSGFV的诊断和防控奠定了基础．
Abstract：Banana streak virus GF(BSGFV)is a major banana streak virus(BSV)that infects bananas.To detect this virus efficiently,a SYBR Green Ⅰ real time fluorescence quantitative PCR(qPCR) method was proposed using a specific primer based on the conserved sequence of the coat protein(CP)gene of BSGFV.The results showed that the optimal concentration of the specific primer BSGFV-qG3F/qG3R of qPCR was 0.2 μmol/L,and the optimal annealing temperature was 59℃ in qPCR detection. The qPCR achieved a detection limit of 1.0×102 copies/μL,100-fold more sensitive than PCR.The coefficient of variation for qPCR Ct values was less than 5%.The qPCR method was suitable for detecting BSGFV in major cultivars like Brazilian bananas(AAA) but unsuitable for hybrids containing the B genome.This study lays the foundation for the diagnosis,prevention,and control of BSGFV in major banana cultivars in the field.
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基本信息:
中图分类号:S436.68
引用信息:
[1]卢咏思,刘润沛,毛自先,等.香蕉线条病毒GF SYBR Green Ⅰ实时荧光定量PCR检测方法的建立[J].仲恺农业工程学院学报,2026,39(01):11-15.
基金信息:
国家现代农业产业技术体系项目(CARS-31)